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KMID : 0545120000100040529
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Journal of Microbiology and Biotechnology
2000 Volume.10 No. 4 p.529 ~ p.531
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Optimized Conditions for In Vitro High Density Encystation of Giardia lamblia
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HONG, WOOK-SUN
KIM, KYONG-JOO/LEE, KISAY
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Abstract
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Giardia lamblia, a waterborne parasitic protozoa causing diarrhea and gastroenteritis, is transmitted to humans from untreated and treated water in the form of cysts. The ingestion of G. lamblia cysts is followed by the excystation of the cysts to trophozoites and subsequent colonization of the upper small intestine. In this study. the in vitro conditions for G. lamblia encystation were investigated to enhance the efficiency of cyst conversion and the resulting cyst density. The trophozoite of G. lamblia was cultivated to the late exponential growth phase, resulting in a high density of over 6×10 exp (7) cells/㎖. The effects of pH, bile content, and induction time were evaluated: A cyst conversion of over 25% and 10 exp (7) cysts/㎖ were routinely obtained using the optimized encystation conditions including a slightly alkaline pH, 10 to 15㎎/㎖ of bile concentration, and 48-50h of induction time.
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